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Corning Life Sciences cell recovery solution corning #354253
Cell Recovery Solution Corning #354253, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+recovery+solution+354253/cell+recovery+solution+corning+354253/pmc09481922__mmc1-3-21-24
Average 90 stars, based on 1 article reviews
cell recovery solution corning #354253 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Cell Recovery:

Article Title: Direct Inhibition of RAS Reveals the Features of Oncogenic Signaling Driven by RAS G12 and Q61 Mutations
Article Snippet: Direct Inhibition of RAS Reveals the Features of Oncogenic Signaling Driven by RAS G12 and Q61 Mutations Michelangelo Marasco1, Dinesh Kumar1, Santiago Garcia Borrego2, Tessa Seale2, Giulia Maddalena3,4,5, Riccardo Mezzadra6, Kylie Belanger2,7,8, Soren Cole2, Brayan Perez2,7,8, Wei Luan6, Radha Mukherjee1, Ilinca Aricescu1, Vladimir Markov9, Yuxin Zhu9, Sabrina Arena10,11, Alberto Bardelli12,13, Elisa de Stanchina9, Scott W. Lowe6, Richard A. Burkhart2,14, Jacquelyn W. Zimmerman2,8, Rona Yaeger15, Scott E. Kopetz3, Neal Rosen1,15, and Sandra Misale2 D ow nloaded from http://aacrjournals.org/cancerdiscovery/article-pdf/doi/10.1158/2159-8290.C D -24-0614/3599494/cd-24-0614.pdf by guest on 01 M ay 2025 AACRJournals.orgOF2 | CANCER DISCOVERY XXX 2025 intRoduction RAS GTPases (KRAS, NRAS, and HRAS) are molecular switches that regulate cell proliferation and survival in response to receptor tyrosine kinase (RTK) activation (1).. In normal cells, RAS proteins alternate between an inactive GDP-bound form and an active GTP-bound form.. RAS cycling is regulated by GTPase-activating proteins (GAP), which catalyze the hydrolysis of RAS-bound GTP, and guanine nucleotide exchange factors, which promote the dissociation of GDP from RAS, allowing its reloading with GTP (2).

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer.
Article Snippet: Female mice (Hsd:Athymic Nude-Foxn1nu, 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/ tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Article Title: A novel strategy based on mouse organoid biosensor for detecting umami substances and their synergistic effect
Article Snippet: Conventional analysis systems face challenges in quantifying the umami perception and synergy within complex food matrices, especially mimicking human taste discrimination.. We thereby developed an umami biosensor based on taste bud organoids and flow cytometry (TOS-FC), which exhibits a broad dynamic range (10 7 to 2 × 10 1 M for monosodium glutamate (MSG)), strong linear correlation (R2 > 98 %), and stable operability beyond sensor lifespan.. The TOS-FC platform successfully quantified three representative umami stimuli-MSG, inosine 5′monophosphate (IMP), and glutamic acid–proline dipeptide (EP, a representative umami peptide).

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer
Article Snippet: Female mice (Hsd:Athymic Nude- Foxn1 nu , 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30 min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer
Article Snippet: Slides are visualized via DAB (BS04-999, Immunologic), counterstained with hematoxylin (105175, Sigma-Aldrich) and mounted using standard procedures according to manufacturer’s protocols. .. Organoids/tumoroids were collected in Cell Recovery Solution (354253, Corning) and placed on ice for 30 min to dissolve Matrigel and subsequently lysed using Laemmli sample buffer (1610737, Bio-Rad, the Netherlands) with Halt protease and phosphatase inhibitor cocktail (1:100, 78440, Thermo Fisher Scientific) and 1.4 M β-mercaptoethanol (M6250, Sigma-Aldrich). .. Protein concentrations were measured with Protein Quantification Assay kit (740967, Bioké) according to manufacturer’s instructions and measured on a BioTek absorbance reader (Agilent).

Article Title: Long-term expansion of basal cells and the novel differentiation methods identify mechanisms for switching Claudin expression in normal epithelia.
Article Snippet: .. For fixation, Matrigel was dissolved in Cell Recovery Solution (354253, Corning) for 1 h at 4 °C. ..

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer.
Article Snippet: Slides are visualized via DAB (BS04-999, Immunologic), counterstained with hematoxylin (105175, Sigma-Aldrich) and mounted using standard procedures according to manufacturer’s protocols. .. Organoids/tumoroids were collected in Cell Recovery Solution (354253, Corning) and placed on ice for 30min to dissolve Matrigel and subsequently lysed using Laemmli sample buffer (1610737, Bio-Rad, the Netherlands) with Halt protease and phosphatase inhibitor cocktail (1:100, 78440, Thermo Fisher Scientific) and 1.4 M β-mercaptoethanol (M6250, Sigma-Aldrich). .. Protein concentrations were measured with Protein Quantification Assay kit (740967, Bioké) according to manufacturer’s instructions and measured on a BioTek absorbance reader (Agilent).

Incubation:

Article Title: Direct Inhibition of RAS Reveals the Features of Oncogenic Signaling Driven by RAS G12 and Q61 Mutations
Article Snippet: Direct Inhibition of RAS Reveals the Features of Oncogenic Signaling Driven by RAS G12 and Q61 Mutations Michelangelo Marasco1, Dinesh Kumar1, Santiago Garcia Borrego2, Tessa Seale2, Giulia Maddalena3,4,5, Riccardo Mezzadra6, Kylie Belanger2,7,8, Soren Cole2, Brayan Perez2,7,8, Wei Luan6, Radha Mukherjee1, Ilinca Aricescu1, Vladimir Markov9, Yuxin Zhu9, Sabrina Arena10,11, Alberto Bardelli12,13, Elisa de Stanchina9, Scott W. Lowe6, Richard A. Burkhart2,14, Jacquelyn W. Zimmerman2,8, Rona Yaeger15, Scott E. Kopetz3, Neal Rosen1,15, and Sandra Misale2 D ow nloaded from http://aacrjournals.org/cancerdiscovery/article-pdf/doi/10.1158/2159-8290.C D -24-0614/3599494/cd-24-0614.pdf by guest on 01 M ay 2025 AACRJournals.orgOF2 | CANCER DISCOVERY XXX 2025 intRoduction RAS GTPases (KRAS, NRAS, and HRAS) are molecular switches that regulate cell proliferation and survival in response to receptor tyrosine kinase (RTK) activation (1).. In normal cells, RAS proteins alternate between an inactive GDP-bound form and an active GTP-bound form.. RAS cycling is regulated by GTPase-activating proteins (GAP), which catalyze the hydrolysis of RAS-bound GTP, and guanine nucleotide exchange factors, which promote the dissociation of GDP from RAS, allowing its reloading with GTP (2).

Transplantation Assay:

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer.
Article Snippet: Female mice (Hsd:Athymic Nude-Foxn1nu, 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/ tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer
Article Snippet: Female mice (Hsd:Athymic Nude- Foxn1 nu , 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30 min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

In Vitro:

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer.
Article Snippet: Female mice (Hsd:Athymic Nude-Foxn1nu, 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/ tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer
Article Snippet: Female mice (Hsd:Athymic Nude- Foxn1 nu , 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30 min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Cell Culture:

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer.
Article Snippet: Female mice (Hsd:Athymic Nude-Foxn1nu, 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/ tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer
Article Snippet: Female mice (Hsd:Athymic Nude- Foxn1 nu , 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30 min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Organoids:

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer.
Article Snippet: Female mice (Hsd:Athymic Nude-Foxn1nu, 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/ tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer
Article Snippet: Female mice (Hsd:Athymic Nude- Foxn1 nu , 6–12 weeks old) were purchased from Envigo and housed in a 12 h light/12 h dark cycle, with temperatures between 20 °C and 24 °C and 40–70% humidity. .. Before transplantation into mice, in vitro cultured organoids/tumoroids were seeded for 72 h in culture medium (as described in section ‘organoid/tumoroid culture’) before harvesting in Cell Recovery Solution (354253, Corning) on ice for 30 min to dissolve the Matrigel. .. To obtain single cells, organoids/tumoroids were harvested in TrypLE (12604013, Thermo Fisher Scientific, the Netherlands) for 3 min at 37 °C.

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer
Article Snippet: Slides are visualized via DAB (BS04-999, Immunologic), counterstained with hematoxylin (105175, Sigma-Aldrich) and mounted using standard procedures according to manufacturer’s protocols. .. Organoids/tumoroids were collected in Cell Recovery Solution (354253, Corning) and placed on ice for 30 min to dissolve Matrigel and subsequently lysed using Laemmli sample buffer (1610737, Bio-Rad, the Netherlands) with Halt protease and phosphatase inhibitor cocktail (1:100, 78440, Thermo Fisher Scientific) and 1.4 M β-mercaptoethanol (M6250, Sigma-Aldrich). .. Protein concentrations were measured with Protein Quantification Assay kit (740967, Bioké) according to manufacturer’s instructions and measured on a BioTek absorbance reader (Agilent).

Article Title: CDX2 downregulation regulates intrinsic WNT pathway activation, dictating metastasis in APC and CTNNB1 wildtype colorectal cancer.
Article Snippet: Slides are visualized via DAB (BS04-999, Immunologic), counterstained with hematoxylin (105175, Sigma-Aldrich) and mounted using standard procedures according to manufacturer’s protocols. .. Organoids/tumoroids were collected in Cell Recovery Solution (354253, Corning) and placed on ice for 30min to dissolve Matrigel and subsequently lysed using Laemmli sample buffer (1610737, Bio-Rad, the Netherlands) with Halt protease and phosphatase inhibitor cocktail (1:100, 78440, Thermo Fisher Scientific) and 1.4 M β-mercaptoethanol (M6250, Sigma-Aldrich). .. Protein concentrations were measured with Protein Quantification Assay kit (740967, Bioké) according to manufacturer’s instructions and measured on a BioTek absorbance reader (Agilent).

other:

Article Title: Metabolic adaptations direct cell fate during tissue regeneration.
Article Snippet: In brief, the day before the experiment, we prepared complete Seahorse medium: Agilent Seahorse XF (pH 7.4) without phenol red and supplemented with glucose (10 mM), pyruvate (1 mM) and glutamine (2 mM).



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